Methodologies used by the authors in the study of biofilms encrusting artifacts, monuments and archaeological sites are illustrated. Techniques adopted in each step of the study, i.e., sampling, identification of biofilm microbial components, analysis of inorganic and organic components of biofilms and removal of biodeteriogens by using radio frequencies are described. A case study of biofilms formed on a substratum in terracotta is presented. Results are discussed by giving special emphasis to the use of radio frequencies for biofilm removal
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